a549  (ATCC)

Journal: iScience

Article Title: Drug screening to identify compounds to eliminate Burkholderia pseudomallei through Hcp protein

doi: 10.1016/j.isci.2026.115367

Figure Lengend Snippet: Netilmicin sulfate alone and in combination exerted protective effects on an infected A549 cell model (A) Cytotoxic effects of the drugs on A549 cells. (B) Protective effects of different concentrations of NETS on A549 cells. The early interventions on the abscissa reflect the incubation of A549 cells with the drug for 2 h before B. pseudomallei HNBP001 infection. Late intervention was defined as the introduction of the drug to A549 cells 2 h after infection with B. pseudomallei HNBP001 . The ordinate is the survival rate of the infected A549 cells. NC: the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Bp+30 μM NETS: The infected A549 cells were treated with 30 μM NETS; the other conditions were the same, but different concentrations of NETS were used. (C) Protective effects of several drugs on infected A549 cells; the ordinate is the survival rate of infected A549 cells. NC, blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Other: The infected A549 cells were treated with drugs (including SXT, GM, NETS, CAZ, and NETS+CAZ). (D) Number of intracellular bacteria in infected A549 cells after drug treatment; the ordinate represents the number of bacteria in infected A549 cells. NC is the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Drug group: 2 × MIC drug was used to treat infected A549 cells. All drugs, including SXT, GM, NETS, CAZ, and NETS+CAZ. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; ns, no significant difference. Data are represented as mean ± SEM.).

Article Snippet: The A549 human lung carcinoma epithelial cell line was purchased from Procell (Wuhan, China; Cat# CL-0016).

Techniques: Infection, Incubation, Negative Control, Bacteria

Journal: Die Naturwissenschaften

Article Title: In vitro antiproliferative activity on A549 and HT-29 cell lines and fatty acid profiling of Agaricus bresadolanus and A. hortensis

doi: 10.1007/s00114-026-02089-0

Figure Lengend Snippet: The antiproliferative activity of the extract of A. bresadolanus on A549 lung cancer cells. Data points represent mean inhibition percentages. Error bars represent mean ± SD from three independent experiments. ( p < 0.01)

Article Snippet: The human lung carcinoma epithelial cell line A549 (ATCC, CCL-185TM), human colorectal adenocarcinoma cell line HT-29 (ATCC, HTB-38TM) and NIH/3T3 fibroblast (ATCC, CRL-1658TM) used in the current work were commercially available.

Techniques: Activity Assay, Inhibition

Journal: Die Naturwissenschaften

Article Title: In vitro antiproliferative activity on A549 and HT-29 cell lines and fatty acid profiling of Agaricus bresadolanus and A. hortensis

doi: 10.1007/s00114-026-02089-0

Figure Lengend Snippet: The antiproliferative activity of the extract of A. hortensis on A549 lung cancer cells. Data points represent mean inhibition percentages. The results given as mean ± SD from three independent experiments. ( p < 0.01)

Article Snippet: The human lung carcinoma epithelial cell line A549 (ATCC, CCL-185TM), human colorectal adenocarcinoma cell line HT-29 (ATCC, HTB-38TM) and NIH/3T3 fibroblast (ATCC, CRL-1658TM) used in the current work were commercially available.

Techniques: Activity Assay, Inhibition

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Cell viability ( A ), LDH release ( B ), and caspase-1 activity ( C ) of the A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and bacteriophages supplemented with 1 mg/mL lactoferrin in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using one-way ANOVA, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Activity Assay, Comparison, Negative Control, Positive Control

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: The CFU/mL of MRSA strains 70 ( A ), 110 ( B ), and 203 ( C ) in infected A549 cell line after treatment with phages vB_SauM-A, vB_SauM-C, and vB_SauM-D, lactoferrin, linezolid, or phage + Lf combination after 3 h and 6 h post-treatment. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using the Kruskal–Wallis test followed by Dunn’s multiple comparison test for values with nonparametric distribution, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Infection, Comparison

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Cell viability ( A , B , C ) and LDH release ( D , E , F ) from the infected A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and phage + Lf combination in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using one-way ANOVA, with *** p < 0.001, ** p < 0.01, and * p < 0.05

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Infection, Comparison, Negative Control, Positive Control

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Inflammasome activation measured by caspase-1 activity in the infected A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and phage + Lf combination in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using the Kruskal–Wallis test followed by Dunn’s multiple comparison test for values with nonparametric distribution, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Activation Assay, Activity Assay, Infection, Comparison, Negative Control, Positive Control